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ProductsBack/NCBP1 Antibody
Western Blot analysis of LOVO cells using CBP80 Polyclonal Antibody
Western Blot analysis of LOVO cells using CBP80 Polyclonal Antibody
Western Blot analysis of LOVO cells using CBP80 Polyclonal Antibody

NCBP1 Antibody

CSB-PA001373
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNCBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NCBP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4686
  • Target name
    NCBP1
  • Target description
    nuclear cap binding protein subunit 1
  • Target synonyms
    CBP80, NCBP, Sto1, nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein, NCBP 80 kDa subunit, nuclear cap binding protein subunit 1, 80kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ09161
  • Protein Name
    Nuclear cap-binding protein subunit 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NCBP1 using anti-NCBP1 antibody (A07424-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCBP1 antigen affinity purified polyclonal antibody (Catalog # A07424-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCBP1 at approximately 92 kDa. The expected band size for NCBP1 is at 92 kDa.
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