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Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: NCF2 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 48, 55, 51 kDa Observed band size: 51, 48 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: NCF2 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 48, 55, 51 kDa Observed band size: 51, 48 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: NCF2 antibody at 2.8microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 48, 55, 51 kDa Observed band size: 51, 48 kDa

NCF2 Antibody

CSB-PA015528LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNCF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NCF2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4688
  • Target name
    NCF2
  • Target description
    neutrophil cytosolic factor 2
  • Target synonyms
    NCF-2, NOXA2, P67-PHOX, P67PHOX, neutrophil cytosol factor 2, 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, chronic granulomatous disease, autosomal 2, neutrophil NADPH oxidase factor 2, neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19878
  • Protein Name
    Neutrophil cytosol factor 2
  • Scientific Description
    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOXA2/p67phox/NCF2 using anti-NOXA2/p67phox/NCF2 antibody (A02476-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOXA2/p67phox/NCF2 antigen affinity purified polyclonal antibody (Catalog # A02476-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOXA2/p67phox/NCF2 at approximately 60KD. The expected band size for NOXA2/p67phox/NCF2 is at 60KD.
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