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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human brain malignant glioma tissue, Primary antibody: CSB-PA993483(NCF2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human brain malignant glioma tissue, Primary antibody: CSB-PA993483(NCF2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: Human brain malignant glioma tissue, Primary antibody: CSB-PA993483(NCF2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes

NCF2 Antibody

CSB-PA993483
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNCF2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NCF2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4688
  • Target name
    NCF2
  • Target description
    neutrophil cytosolic factor 2
  • Target synonyms
    67 kDa neutrophil oxidase factor; chronic granulomatous disease, autosomal 2; NADPH oxidase activator 2; NCF-2; neutrophil cytosol factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); neutrophil NADPH oxidase factor 2; NOXA2; P67PHOX; P67-PHOX
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19878
  • Protein Name
    Neutrophil cytosol factor 2
  • Scientific Description
    This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NOXA2/p67phox/NCF2 using anti-NOXA2/p67phox/NCF2 antibody (A02476-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOXA2/p67phox/NCF2 antigen affinity purified polyclonal antibody (Catalog # A02476-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOXA2/p67phox/NCF2 at approximately 60KD. The expected band size for NOXA2/p67phox/NCF2 is at 60KD.
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