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NCF2 / NOXA2 / p67phox Antibody

LS-C17141
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNCF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NCF2 / NOXA2 / p67phox Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:500-1:2000 detects p67-phox in RIPA lysates from HL-60 cells. Note: p67-phox is highly susceptible to proteases. Immunoprecipitation: 4 ul immunoprecipitated p67-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:500-1:4000 shows positive cytosolic immunostaining for p67-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.. ICC (1:500 - 1:4000), IP, WB (1:500 - 1:2000) Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Western Blot: 1:500-1:2000 detects p67-phox in RIPA lysates from HL-60 cells. Note: p67-phox is highly susceptible to proteases. Immunoprecipitation: 4 ul immunoprecipitated p67-phox from 500 ug of HL-60 RIPA lysate. Immunocytochemistry: 1:500-1:4000 shows positive cytosolic immunostaining for p67-phox in HL-60 cells fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4688
  • Target name
    NCF2
  • Target description
    neutrophil cytosolic factor 2
  • Target synonyms
    67 kDa neutrophil oxidase factor; chronic granulomatous disease, autosomal 2; NADPH oxidase activator 2; NCF-2; neutrophil cytosol factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2); neutrophil NADPH oxidase factor 2; NOXA2; P67PHOX; P67-PHOX
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NOXA2/p67phox/NCF2 using anti-NOXA2/p67phox/NCF2 antibody (A02476-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOXA2/p67phox/NCF2 antigen affinity purified polyclonal antibody (Catalog # A02476-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOXA2/p67phox/NCF2 at approximately 60KD. The expected band size for NOXA2/p67phox/NCF2 is at 60KD.
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