ProductsBack/NCOR2 Antibody

NCOR2 Antibody

CAB8388

TargetNCOR2

Product group Antibodies

Overview

Supplier
Assay Genie
Product Name
NCOR2 Rabbit pAb
Delivery Days Customer
9
Applications Supplier
WB,IHC,IF
Certification
Research Use Only
Clonality
Polyclonal
Gene ID9612
Target name
NCOR2
Target description
nuclear receptor corepressor 2
Target synonyms
CTG repeat protein 26; CTG26; N-CoR2; nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; SMAP270; SMRT; SMRTE; SMRTE-tau; T3 receptor-associating factor; thyroid-, retinoic-acid-receptor-associated corepressor; TNRC14; TRAC; TRAC1; TRAC-1
Host
Rabbit
Protein IDQ9Y618
Protein Name
Nuclear receptor corepressor 2
Shelf life instruction
12 months
Source
Rabbit
Reactivity Supplier
Human,Mouse,Rat
Storage Instruction
-20°C
UNSPSC
12352203

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Figure 1. Western blot analysis of NCOR2 using anti-NCOR2 antibody (A01412-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOR2 antigen affinity purified polyclonal antibody (Catalog # A01412-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOR2 at approximately 275 kDa. The expected band size for NCOR2 is at 275 kDa.

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