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NDRG4 antibody

ORB543146
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNDRG4
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Overview

  • Supplier
    Biorbyt
  • Product Name
    NDRG4 antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    WB: 1:500-1:2000 ELISA, IF, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65009
  • Target name
    NDRG4
  • Target description
    NDRG family member 4
  • Target synonyms
    BDM1, SMAP-8, SMAP8, protein NDRG4, N-myc downstream-regulated gene 4 protein, brain development-related molecule 1, smooth muscle-associated protein 8, vascular smooth muscle cell-associated protein 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULP0
  • Protein Name
    Protein NDRG4
  • Scientific Description
    Rabbit polyclonal antibody to NDRG4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDRG4 using anti-NDRG4 antibody (A04972-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDRG4 antigen affinity purified polyclonal antibody (Catalog # A04972-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDRG4 at approximately 37 kDa. The expected band size for NDRG4 is at 37-45 kDa.
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