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Western Blot analysis of RAW264.7 cells using NDUFA4 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using NDUFA4 Polyclonal Antibody
Western Blot analysis of RAW264.7 cells using NDUFA4 Polyclonal Antibody

NDUFA4 Antibody

CSB-PA003384
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCOXFA4
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Overview

  • Supplier
    Cusabio
  • Product Name
    NDUFA4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4697
  • Target name
    COXFA4
  • Target description
    cytochrome c oxidase associated subunit FA4
  • Target synonyms
    CI-9k, CI-MLRQ, MC4DN21, MISTR1, MLRQ, MRCAF1, NDUFA4, cytochrome c oxidase subunit NDUFA4, Complex I 9kDa subunit, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4, NADH-ubiquinone oxidoreductase MLRQ subunit, NDUFA4 mitochondrial complex associated, complex I-MLRQ, cytochrome c oxidase subunit FA4, mitochondrial respiratory chain associated factor 1, mitochondrial stress response 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00483
  • Protein Name
    Cytochrome c oxidase subunit NDUFA4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFA4 using anti-NDUFA4 antibody (A09276-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T98G whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA4 antigen affinity purified polyclonal antibody (Catalog # A09276-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA4 at approximately 12 kDa. The expected band size for NDUFA4 is at 9 kDa.
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