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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA795585(NDUFA8 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA795585(NDUFA8 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA795585(NDUFA8 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)

NDUFA8 Antibody

CSB-PA795585
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNDUFA8
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Overview

  • Supplier
    Cusabio
  • Product Name
    NDUFA8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4702
  • Target name
    NDUFA8
  • Target description
    NADH:ubiquinone oxidoreductase subunit A8
  • Target synonyms
    CI-19KD; CI-PGIV; complex I-19kD; complex I-PGIV; MC1DN37; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; NADH:ubiquinone oxidoreductase PGIV subunit; NADH-ubiquinone oxidoreductase 19 kDa subunit; PGIV
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51970
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
  • Scientific Description
    The protein encoded by this gene belongs to the complex I 19 kDA subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFA8 using anti-NDUFA8 antibody (A10547-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA8 antigen affinity purified polyclonal antibody (Catalog # A10547-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA8 at approximately 19 kDa. The expected band size for NDUFA8 is at 20 kDa.
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