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IP analysis of 293T cell lysate using GTX65622 NDUFA8 antibody. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000
IP analysis of 293T cell lysate using GTX65622 NDUFA8 antibody. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000
IP analysis of 293T cell lysate using GTX65622 NDUFA8 antibody. Antibody amount : 3microg / 200microg lysate Dilution : 1:1000

NDUFA8 antibody

GTX65622
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetNDUFA8
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Overview

  • Supplier
    GeneTex
  • Product Name
    NDUFA8 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IP: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4702
  • Target name
    NDUFA8
  • Target description
    NADH:ubiquinone oxidoreductase subunit A8
  • Target synonyms
    CI-19KD; CI-PGIV; complex I-19kD; complex I-PGIV; MC1DN37; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; NADH:ubiquinone oxidoreductase PGIV subunit; NADH-ubiquinone oxidoreductase 19 kDa subunit; PGIV
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51970
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8
  • Scientific Description
    The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFA8 using anti-NDUFA8 antibody (A10547-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA8 antigen affinity purified polyclonal antibody (Catalog # A10547-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA8 at approximately 19 kDa. The expected band size for NDUFA8 is at 20 kDa.
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