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ProductsBack/NDUFB10 antibody [N1C3]

NDUFB10 antibody [N1C3]

GTX114576
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNDUFB10
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Overview

  • Supplier
    GeneTex
  • Product Name
    NDUFB10 antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4716
  • Target name
    NDUFB10
  • Target description
    NADH:ubiquinone oxidoreductase subunit B10
  • Target synonyms
    MC1DN35, PDSW, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10, CI-PDSW, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa, NADH ubiquinone oxidoreductase PDSW subunit (RH 16p13.3), NADH-ubiquinone oxidoreductase PDSW subunit, complex I PDSW subunit, complex I-PDSW
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO96000
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of NDUFB10 using anti-NDUFB10 antibody (A11886-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: human HL-60 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB10 antigen affinity purified polyclonal antibody (Catalog # A11886-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB10 at approximately 22KD. The expected band size for NDUFB10 is at 22KD.
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