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NDUFB3 antibody

GTX111211
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetNDUFB3
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Overview

  • Supplier
    GeneTex
  • Product Name
    NDUFB3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4709
  • Target name
    NDUFB3
  • Target description
    NADH:ubiquinone oxidoreductase subunit B3
  • Target synonyms
    B12, CI-B12, MC1DN25, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa, NADH-ubiquinone oxidoreductase B12 subunit, complex I-B12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43676
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
  • Scientific Description
    The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. See NDUFA2 (MIM 602137).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFB3 using anti-NDUFB3 antibody (A12461-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB3 antigen affinity purified polyclonal antibody (Catalog # A12461-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB3 at approximately 14KD. The expected band size for NDUFB3 is at 14KD.
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