More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/NDUFB4 Polyclonal Antibody
L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

NDUFB4 Polyclonal Antibody

BS-55145R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNDUFB4
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    NDUFB4 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:400-800), IF(IF=1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4710
  • Target name
    NDUFB4
  • Target description
    NADH:ubiquinone oxidoreductase subunit B4
  • Target synonyms
    B15; CI-B15; complex I B15 subunit; complex I-B15; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; NADH-ubiquinone oxidoreductase B15 subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95168
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Figure 1. Western blot analysis of NDUFB4 using anti-NDUFB4 antibody (A11016-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB4 antigen affinity purified polyclonal antibody (Catalog # A11016-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB4 at approximately 15 kDa. The expected band size for NDUFB4 is at 15 kDa.
Product group Antibodies
Boster Bio
Anti-NDUFB4 Antibody Picoband(r)A11016-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetNDUFB4
  • SizePrice
Anti-NDUFB4 Antibody
Product group Antibodies
Antibodies.com
Anti-NDUFB4 AntibodyA92828
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NDUFB4 Antibody144-60491
ApplicationsWestern Blot
TargetNDUFB4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
NDUFB4 / B15 AntibodyLS-C748843
ApplicationsWestern Blot
TargetNDUFB4
  • SizePrice
Product group Antibodies
GeneTex
NDUFB4 antibodyGTX111026
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetNDUFB4
  • SizePrice
Immunohistochemistry analysis in human heart muscle and pancreas tissues using Anti-NDUFB4 antibody. Corresponding NDUFB4 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-NDUFB4 AntibodyHPA051739
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetNDUFB4
  • SizePrice
Product group Antibodies
MedChem Express
NDUFB4 Antibody (YA1338, Rabbit)HY-P81593
TargetNDUFB4
  • SizePrice