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NDUFB5 Antibody

LS-C816797
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNDUFB5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NDUFB5 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:100 - 1:500), IF (1:100 - 1:500), IHC-P (1:50 - 1:200) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4711
  • Target name
    NDUFB5
  • Target description
    NADH:ubiquinone oxidoreductase subunit B5
  • Target synonyms
    CISGDH, SGDH, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa, NADH-ubiquinone oxidoreductase SGDH subunit, complex I SGDH subunit, complex I-SGDH
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: NDUFB5 antibody at 2.64microg/ml Lane 1: Mouse heart tissue Lane 2: Mouse kidney tissue Lane 3: 293T whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22, 16 kDa Observed band size: 16 kDa
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Figure 1. Western blot analysis of NDUFB5 using anti-NDUFB5 antibody (A10585-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: rat liver tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse kidney tissue lysates, Lane 10: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB5 antigen affinity purified polyclonal antibody (Catalog # A10585-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB5 at approximately 22KD. The expected band size for NDUFB5 is at 16KD.
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Immunohistochemistry analysis in human heart muscle and pancreas tissues using HPA005640 antibody. Corresponding NDUFB5 RNA-seq data are presented for the same tissues.
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