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NDUFB8 Antibody (YA1947)

HY-P82202
MedChem Express
TargetNDUFB8
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    NDUFB8 Antibody (YA1947)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4714
  • Target name
    NDUFB8
  • Target description
    NADH:ubiquinone oxidoreductase subunit B8
  • Target synonyms
    ASHI; CI-ASHI; complex I ASHI subunit; complex I-ASHI; MC1DN32; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; NADH:ubiquinone oxidoreductase ASHI subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    NDUFB8 Antibody (YA1947) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1947), targeting NDUFB8, with a predicted molecular weight of 22 kDa (observed band size: 19 kDa). NDUFB8 Antibody (YA1947) can be used for WB, IHC-P, ICC/IF, IP, FC experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFB8 using anti-NDUFB8 antibody (A07936-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human U937 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human HL-60 whole cell lysates, Lane 9: rat liver tissue lysates, Lane 10: rat heart tissue lysates, Lane 11: mouse liver tissue lysates, Lane 12: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB8 antigen affinity purified polyclonal antibody (Catalog # A07936-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB8 at approximately 19-22 kDa. The expected band size for NDUFB8 is at 22 kDa.
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