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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA064548(NDUFS5 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA064548(NDUFS5 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA064548(NDUFS5 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: x200)

NDUFS5 Antibody

CSB-PA064548
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNDUFS5
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Overview

  • Supplier
    Cusabio
  • Product Name
    NDUFS5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4725
  • Target name
    NDUFS5
  • Target description
    NADH:ubiquinone oxidoreductase subunit S5
  • Target synonyms
    CI-15k, CI15K, NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, CI-15 kDa, NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase), NADH:ubiquinone oxidoreductase 15 kDa IP subunit, complex I-15 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43920
  • Protein Name
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
  • Scientific Description
    This gene is a member of the NADH dehydrogenase (ubiquinone) iron-sulfur protein family. The encoded protein is a subunit of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. Alternative splicing results in multiple transcript variants and pseudogenes have been identified on chromosomes 1, 4 and 17.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFS5 using anti-NDUFS5 antibody (A04093-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS5 antigen affinity purified polyclonal antibody (Catalog # A04093-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS5 at approximately 15 kDa. The expected band size for NDUFS5 is at 13 kDa.
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