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NDUFS5 Antibody Pair

CSB-EAP02735
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityChicken, Equine, Guinea Pig, Goat, Human
TargetNDUFS5
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Overview

  • Supplier
    Cusabio
  • Product Name
    NDUFS5 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.34ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID4725
  • Target name
    NDUFS5
  • Target description
    NADH:ubiquinone oxidoreductase subunit S5
  • Target synonyms
    CI-15k, CI15K, NADH dehydrogenase [ubiquinone] iron-sulfur protein 5, CI-15 kDa, NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa (NADH-coenzyme Q reductase), NADH:ubiquinone oxidoreductase 15 kDa IP subunit, complex I-15 kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43920
  • Protein Name
    NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
  • Reactivity
    Chicken, Equine, Guinea Pig, Goat, Human
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of NDUFS5 using anti-NDUFS5 antibody (A04093-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFS5 antigen affinity purified polyclonal antibody (Catalog # A04093-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFS5 at approximately 15 kDa. The expected band size for NDUFS5 is at 13 kDa.
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