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NECAB2 Antibody

CSB-PA015675GA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNECAB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NECAB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54550
  • Target name
    NECAB2
  • Target description
    N-terminal EF-hand calcium binding protein 2
  • Target synonyms
    EFCBP2; EF-hand calcium-binding protein 2; neuronal calcium binding 2; neuronal calcium-binding protein 2; N-terminal EF-hand calcium-binding protein 2; stip-2; synaptotagmin-interacting protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z6G3
  • Protein Name
    N-terminal EF-hand calcium-binding protein 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NECAB2 using anti-NECAB2 antibody (A13886-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB2 antigen affinity purified polyclonal antibody (Catalog # A13886-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NECAB2 at approximately 43 kDa. The expected band size for NECAB2 is at 43 kDa.
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