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Western blot All lanes: NEDD1 antibody at 3.25ug/ml Lane 1: Mouse small intestine tissue Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72, 63, 73 kDa Observed band size: 72 kDa
Western blot All lanes: NEDD1 antibody at 3.25ug/ml Lane 1: Mouse small intestine tissue Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72, 63, 73 kDa Observed band size: 72 kDa
Western blot All lanes: NEDD1 antibody at 3.25ug/ml Lane 1: Mouse small intestine tissue Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 72, 63, 73 kDa Observed band size: 72 kDa

NEDD1 Antibody

CSB-PA822820DSR1HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNEDD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NEDD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID121441
  • Target name
    NEDD1
  • Target description
    NEDD1 gamma-tubulin ring complex targeting factor
  • Target synonyms
    GCP-WD, TUBGCP7, protein NEDD1, NEDD-1, neural precursor cell expressed developmentally down-regulated protein 1, neural precursor cell expressed, developmentally down-regulated 1, tubulin gamma complex associated protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NHV4
  • Protein Name
    Protein NEDD1
  • Scientific Description
    Required for mitosis progression. Promotes the nucleation of microtubules from the spindle.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NEDD1 using anti-NEDD1 antibody (A06427-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD1 antigen affinity purified polyclonal antibody (Catalog # A06427-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD1 at approximately 70 kDa. The expected band size for NEDD1 is at 70 kDa.
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