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NEDD4-2 Recombinant Antibody

BSM-61294R
Bioss Antibodies
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNEDD4L
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    NEDD4-2 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    WB(1:300-5000), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID23327
  • Target name
    NEDD4L
  • Target description
    NEDD4 like E3 ubiquitin protein ligase
  • Target synonyms
    E3 ubiquitin-protein ligase NEDD4-like; HECT-type E3 ubiquitin transferase NED4L; hNEDD4-2; NEDD4.2; NEDD4-2; neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase; PVNH7; RSP5; ubiquitin-protein ligase Rsp5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PU5
  • Protein Name
    E3 ubiquitin-protein ligase NEDD4-like
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NEDD4-2 using anti-NEDD4-2 antibody (A01595-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: mouse pancreas tissue lysates, Lane 5: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD4-2 antigen affinity purified polyclonal antibody (Catalog # A01595-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD4-2 at approximately 130KD. The expected band size for NEDD4-2 is at 112KD.
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