Bio-Connect
NEDD8-AMC (human) (rec.) (untagged)

NEDD8-AMC (human) (rec.) (untagged)

SBB-PS0044
South Bay Bio
Protein IDQ15843
Product group Proteins / Signaling Molecules
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    South Bay Bio
  • Product Name
    NEDD8-AMC (human) (rec.) (untagged)
  • Delivery Days Customer
    10
  • Estimated Purity
    >97%
  • Protein IDQ15843
  • Protein Name
    NEDD8
  • Scientific Description
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the c-terimnus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rh 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome and NEDP1. This product consists of a full-length, mature NEDD8 polypeptide (amino acids 1-76) recombinantly expressed in E.coli, conjugated on its C-terminus to a quenched AMC110 dye. - Recombinant Protein. Full-length, mature NEDD8 polypeptide conjugated on its C-terminus to AMC. Source/Host: E. coli. Liquid. In 50mM MES pH 6.0, 150mM sodium chloride. NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the c-terimnus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rh 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome and NEDP1. This product consists of a full-length, mature NEDD8 polypeptide (amino acids 1-76) recombinantly expressed in E.coli, conjugated on its C-terminus to a quenched AMC110 dye.
  • Storage Instruction
    -80°C
  • UNSPSC
    12352202

Related products

Signal to Background: The signal to background ratio was determined by 100% hydrolysis of 800nM, 400nM, 200nM, 100nM, 50nM NEDD8-Rhodamine 110 to liberate the quenched conjugate. Assay Buffer: 50mM HEPES pH7.5, 1mM TCEP, 0.1mg/ml BSA.
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDQ15843
  • SizePrice
Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 17.5nM and 0.1nM Ubiquitin Aldehyde (SBB-PS0031).
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
  • SizePrice
Kinetic Activity: UCHL3 (SBB-DE0023) activity with Ubiquitin-AMC (SBB-PS0043) measured in the presence of 12.5nM and 0.1nM Ubiquitin vinyl sulfone (SBB-PS0032).
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
  • SizePrice
Ubiquitin-AMC (human) (rec.) (untagged)
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
  • SizePrice
NEDD8-AMC (human) (rec.) (untagged)
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDQ15843
  • SizePrice
HA-Ubiquitin-vinyl sulfone (human) (rec.) (HA)
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
  • SizePrice
HA-Ubiquitin-vinyl methyl ester (human) (rec.) (HA)
Product group Proteins / Signaling Molecules
South Bay Bio
Protein IDP0CG47
  • SizePrice