More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/NEDD8 antibody
WB analysis of various sample lysates using GTX54567 NEDD8 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54567 NEDD8 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX54567 NEDD8 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane

NEDD8 antibody

GTX54567
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNEDD8
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    NEDD8 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4738
  • Target name
    NEDD8
  • Target description
    NEDD8 ubiquitin like modifier
  • Target synonyms
    NEDD-8, ubiquitin-like protein NEDD8, neddylin, neural precursor cell expressed, developmentally down-regulated 8, ubiquitin-like protein Nedd8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15843
  • Protein Name
    NEDD8
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • NEDDylation negatively regulates ERRbeta expression to promote breast cancer tumorigenesis and progression. Naik SK et al., 2020 Aug 24, Cell Death Dis
    Read more

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot analysis of HepG2 cells using Neddylin Polyclonal Antibody
Product group Antibodies
Cusabio
NEDD8 AntibodyCSB-PA003391
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetNEDD8
  • SizePrice
Product group Antibodies
Biomatik
Nedd8 Polyclonal AntibodyCAC10602
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetNEDD8
  • SizePrice
Product group Antibodies
GeneTex
NEDD8 antibody [ND23]GTX30772
ApplicationsWestern Blot
TargetNEDD8
  • SizePrice
ELISA analysis of antigen using GTX60545 NEDD8 antibody [1A7]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
Product group Antibodies
GeneTex
NEDD8 antibody [1A7]GTX60545
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetNEDD8
  • SizePrice
ELISA analysis of antigen using GTX60546 NEDD8 antibody [5B8]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
Product group Antibodies
GeneTex
NEDD8 antibody [5B8]GTX60546
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetNEDD8
  • SizePrice
Product group Antibodies
MedChem Express
NEDD8 Antibody (YA273, Rabbit)HY-P80240
TargetNEDD8
  • SizePrice
Anti-NEDD8 Antibody
Product group Antibodies
Antibodies.com
Anti-NEDD8 AntibodyA95825
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Figure 1. Western blot analysis of NEDD8 using anti-NEDD8 antibody (A00547). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD8 antigen affinity purified polyclonal antibody (Catalog # A00547) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD8 at approximately 10-12 kDa. The expected band size for NEDD8 is at 9 kDa.
Product group Antibodies
Boster Bio
Anti-NEDD8 Antibody Picoband(r)A00547-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetNEDD8
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NEDD8 Antibody144-01163
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetNEDD8
  • SizePrice
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
Anti-NEDD8 AntibodyHPA027583
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetNEDD8
  • SizePrice