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ProductsBack/NEDD8 Mouse Monoclonal Antibody [Clone ID: 5B8]
Western blot analysis using NEDD8 antibody against human NEDD8 (AA: 1-81) recombinant protein (Expected MW is 40 kDa).
Western blot analysis using NEDD8 antibody against human NEDD8 (AA: 1-81) recombinant protein (Expected MW is 40 kDa).
Western blot analysis using NEDD8 antibody against human NEDD8 (AA: 1-81) recombinant protein (Expected MW is 40 kDa).

NEDD8 Mouse Monoclonal Antibody [Clone ID: 5B8]

AM06719PU-N
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNEDD8
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    NEDD8 Mouse Monoclonal Antibody [Clone ID: 5B8]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B8
  • Gene ID4738
  • Target name
    NEDD8
  • Target description
    NEDD8 ubiquitin like modifier
  • Target synonyms
    NEDD-8, ubiquitin-like protein NEDD8, neddylin, neural precursor cell expressed, developmentally down-regulated 8, ubiquitin-like protein Nedd8
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15843
  • Protein Name
    Ubiquitin-like protein NEDD8
  • Scientific Description
    NEDD8 mouse monoclonal antibody, clone 5B8, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of NEDD8 using anti-NEDD8 antibody (A00547). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEDD8 antigen affinity purified polyclonal antibody (Catalog # A00547) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEDD8 at approximately 10-12 kDa. The expected band size for NEDD8 is at 9 kDa.
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