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NEDD9 Antibody (YA4268, Mouse)

HY-P84571
MedChem Express
TargetNEDD9
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    NEDD9 Antibody (YA4268, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4739
  • Target name
    NEDD9
  • Target description
    neural precursor cell expressed, developmentally down-regulated 9
  • Target synonyms
    CAS-L, CAS2, CASL, CASS2, HEF1, enhancer of filamentation 1, Cas scaffolding protein family member 2, Crk-associated substrate related protein Cas-L, Enhancer of filamentation 1 p55, cas-like docking, neural precursor cell expressed developmentally down-regulated protein 9, p130Cas-related protein, renal carcinoma antigen NY-REN-12
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    NEDD9 Antibody (YA4268) is a mouse-derived and non-conjugated IgG1 antibody, targeting to NEDD9. It can be applicated for WB, ICC/IF, FC, ELISA assays, in the background of human, rat.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HEF1 using anti-HEF1 antibody (PB9289). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HEF1 antigen affinity purified polyclonal antibody (Catalog # PB9289) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HEF1 at approximately 93-105 kDa. The expected band size for HEF1 is at 93 kDa.
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