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Western Blot analysis of COLO205 cells using NEGR1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using NEGR1 Polyclonal Antibody
Western Blot analysis of COLO205 cells using NEGR1 Polyclonal Antibody

NEGR1 Antibody

CSB-PA003394
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNEGR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NEGR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID257194
  • Target name
    NEGR1
  • Target description
    neuronal growth regulator 1
  • Target synonyms
    DMML2433, IGLON4, KILON, Ntra, neuronal growth regulator 1, IgLON family member 4, a kindred of IgLON, neurotractin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z3B1
  • Protein Name
    Neuronal growth regulator 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NEGR1 using anti-NEGR1 antibody (A06730-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEGR1 antigen affinity purified polyclonal antibody (Catalog # A06730-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEGR1 at approximately 46 kDa. The expected band size for NEGR1 is at 39 kDa.
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