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NELFE Antibody (YA1365)

HY-P81620
MedChem Express
TargetNELFE
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    NELFE Antibody (YA1365)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID7936
  • Target name
    NELFE
  • Target description
    negative elongation factor complex member E
  • Target synonyms
    D6S45; major histocompatibility complex gene RD; negative elongation factor E; negative elongation factor polypeptide E; NELF-E; nuclear protein; RD; RD RNA-binding protein; RDBP; RDP; RNA-binding protein RD
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    NELFE Antibody (YA1365) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1365), targeting NELFE, with a predicted molecular weight of 43 kDa (observed band size: 43 kDa). NELFE Antibody (YA1365) can be used for WB, IHC-P, IP experiment in human, mouse, rat background.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NELFE using anti-NELFE antibody (A07050-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NELFE antigen affinity purified polyclonal antibody (Catalog # A07050-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NELFE at approximately 43 kDa. The expected band size for NELFE is at 43 kDa.
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