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Rabbit anti-NELFE Antibody, Affinity Purified

A301-913A
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetNELFE
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Overview

  • Supplier
    Bethyl Laboratories
  • Product Name
    Rabbit anti-NELFE Antibody, Affinity Purified
  • Delivery Days Customer
    9
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1000 ug/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7936
  • Target name
    NELFE
  • Target description
    negative elongation factor complex member E
  • Target synonyms
    D6S45, NELF-E, RD, RDBP, RDP, negative elongation factor E, RD RNA-binding protein, RNA-binding protein RD, major histocompatibility complex gene RD, negative elongation factor polypeptide E, nuclear protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18615
  • Protein Name
    Negative elongation factor E
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NELFE using anti-NELFE antibody (A07050-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NELFE antigen affinity purified polyclonal antibody (Catalog # A07050-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NELFE at approximately 43 kDa. The expected band size for NELFE is at 43 kDa.
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