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Nephrin Antibody (YA3472)

HY-P83743
MedChem Express
TargetNPHS1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Nephrin Antibody (YA3472)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Mix
  • Gene ID4868
  • Target name
    NPHS1
  • Target description
    NPHS1 adhesion molecule, nephrin
  • Target synonyms
    CNF; nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHN; NPHS1, nephrin; renal glomerulus-specific cell adhesion receptor; truncated NPHS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Nephrin Antibody (YA3472) is a non-conjugated IgG antibody, targeting Nephrin, with a predicted molecular weight of 135 kDa (observed band size: 200 kDa). Nephrin Antibody (YA3472) can be used for WB, IHC-P, IF-Tissue, IHC-Fr experiment in human, Mouse and Rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA015988LA01HU diluted at 1:250 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of Nephrin using anti-Nephrin antibody (A01991). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nephrin antigen affinity purified polyclonal antibody (Catalog # A01991) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nephrin at approximately 185 kDa. The expected band size for Nephrin is at 135 kDa.
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