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Rat RSC96 cells probed with Nephrin Polyclonal Antibody, Unconjugated (bs-10233R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Rat RSC96 cells probed with Nephrin Polyclonal Antibody, Unconjugated (bs-10233R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Rat RSC96 cells probed with Nephrin Polyclonal Antibody, Unconjugated (bs-10233R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

Nephrin Polyclonal Antibody

BS-10233R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNPHS1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Nephrin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500), Flow-Cyt(Flow-Cyt=1μg/Test)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4868
  • Target name
    NPHS1
  • Target description
    NPHS1 adhesion molecule, nephrin
  • Target synonyms
    CNF; nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHN; NPHS1, nephrin; renal glomerulus-specific cell adhesion receptor; truncated NPHS1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60500
  • Protein Name
    Nephrin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Combination therapy of exendin-4 and allogenic adipose-derived mesenchymal stem cell preserved renal function in a chronic kidney disease and sepsis syndrome setting in rats. Chen CH et al., 2017 Nov 21, Oncotarget
    Read more

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Figure 1. Western blot analysis of Nephrin using anti-Nephrin antibody (A01991). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nephrin antigen affinity purified polyclonal antibody (Catalog # A01991) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nephrin at approximately 185 kDa. The expected band size for Nephrin is at 135 kDa.
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