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NETO2 Antibody (aa174-203)

LS-C323298
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNETO2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NETO2 Antibody (aa174-203)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:1000), Flo (1:10 - 1:50), IHC (1:10 - 1:50), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID81831
  • Target name
    NETO2
  • Target description
    neuropilin and tolloid like 2
  • Target synonyms
    brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2; BTCL2; NEOT2; neuropilin (NRP) and tolloid (TLL)-like 2; neuropilin and tolloid-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NETO2 using anti-NETO2 antibody (A09555-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NETO2 antigen affinity purified polyclonal antibody (Catalog # A09555-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NETO2 at approximately 65 kDa. The expected band size for NETO2 is at 59 kDa.
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