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Western blot All lanes: NETO2 antibody at 4microg/ml Lane 1: U251 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 23, 59 kDa Observed band size: 70 kDa
Western blot All lanes: NETO2 antibody at 4microg/ml Lane 1: U251 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 23, 59 kDa Observed band size: 70 kDa
Western blot All lanes: NETO2 antibody at 4microg/ml Lane 1: U251 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 23, 59 kDa Observed band size: 70 kDa

NETO2 Antibody

CSB-PA818759LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNETO2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NETO2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81831
  • Target name
    NETO2
  • Target description
    neuropilin and tolloid like 2
  • Target synonyms
    BTCL2, NEOT2, neuropilin and tolloid-like protein 2, brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 2, neuropilin (NRP) and tolloid (TLL)-like 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NC67
  • Protein Name
    Neuropilin and tolloid-like protein 2
  • Scientific Description
    Accessory subunit of neuronal kainate-sensitive glutamate receptors, GRIK2 and GRIK3. Increases kainate-receptor channel activity, slowing the decay kinetics of the receptors, without affecting their expression at the cell surface, and increasing the open probability of the receptor channels. Modulates the agonist sensitivity of kainate receptors. Slows the decay of kainate receptor-mediated excitatory postsynaptic currents (EPSCs), thus directly influencing synaptic transmission.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NETO2 using anti-NETO2 antibody (A09555-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SIHA whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NETO2 antigen affinity purified polyclonal antibody (Catalog # A09555-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NETO2 at approximately 65 kDa. The expected band size for NETO2 is at 59 kDa.
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