Neutrophil elastase antibody [NP57]

GeneTex

Neutrophil elastase antibody [NP57]

9

IHC: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:150 with AutoProbe III (GTX28620). Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies. The optimal conditions should be determined by the individual laboratory.

ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

NP57

0.1 mg/ml

Unconjugated

Liquid

ELANE

elastase, neutrophil expressed

bone marrow serine protease; ELA2; elastase 2, neutrophil; elastase-2; GE; granulocyte-derived elastase; HLE; HNE; leukocyte elastase; medullasin; NE; neutrophil elastase; PMN elastase; PMN-E; polymorphonuclear elastase; polymorphonuclear leukocyte elastase; SCN1

Mouse

IgG1

Neutrophil elastase

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016]

Human

-20°C or -80°C,2°C to 8°C

12352203