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ProductsBack/Neutrophil Elastase (ELANE) Rabbit mAb

Neutrophil Elastase (ELANE) Rabbit mAb

A8953
ABclonal
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetELANE
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Overview

  • Supplier
    ABclonal
  • Product Name
    Neutrophil Elastase (ELANE) Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID1991
  • Target name
    ELANE
  • Target description
    elastase, neutrophil expressed
  • Target synonyms
    ELA2, GE, HLE, HNE, NE, PMN-E, SCN1, neutrophil elastase, PMN elastase, bone marrow serine protease, elastase 2, neutrophil, elastase-2, granulocyte-derived elastase, leukocyte elastase, medullasin, polymorphonuclear elastase, polymorphonuclear leukocyte elastase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08246
  • Protein Name
    Neutrophil elastase
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Neutrophil Elastase using anti-Neutrophil Elastase antibody (M01607). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neutrophil Elastase antigen affinity purified monoclonal antibody (Catalog # M01607) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neutrophil Elastase at approximately 29 kDa. The expected band size for Neutrophil Elastase is at 29 kDa.
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