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NF2 Antibody (YA1386, Rabbit)

HY-P81641
MedChem Express
TargetNF2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    NF2 Antibody (YA1386, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4771
  • Target name
    NF2
  • Target description
    neurofibromin 2
  • Target synonyms
    ACN; BANF; bilateral acoustic neurofibromatosis; merlin; merlin-1; moesin-ezrin-radixin like; moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromin 2 (bilateral acoustic neuroma); SCH; schwannomerlin; schwannomin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    NF2 Antibody (YA1386) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1386), targeting NF2, with a predicted molecular weight of 70 kDa (observed band size: 70 kDa). NF2 Antibody (YA1386) can be used for WB, IP experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NF2/Merlin using anti-NF2/Merlin antibody (A00279-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF2/Merlin antigen affinity purified polyclonal antibody (Catalog # A00279-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF2/Merlin at approximately 69 kDa. The expected band size for NF2/Merlin is at 70 kDa.
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