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Western Blot analysis of COLO205 cells using NFATc4 Polyclonal Antibody
Western Blot analysis of COLO205 cells using NFATc4 Polyclonal Antibody
Western Blot analysis of COLO205 cells using NFATc4 Polyclonal Antibody

NFATC4 Antibody

CSB-PA003422
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNFATC4
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Overview

  • Supplier
    Cusabio
  • Product Name
    NFATC4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4776
  • Target name
    NFATC4
  • Target description
    nuclear factor of activated T cells 4
  • Target synonyms
    NF-AT3, NF-ATC4, NFAT3, nuclear factor of activated T-cells, cytoplasmic 4, T-cell transcription factor NFAT3, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14934
  • Protein Name
    Nuclear factor of activated T-cells, cytoplasmic 4
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NFATC4 using anti-NFATC4 antibody (A02599-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates, Lane 3: human Hela whole cell lysates, Lane 4: human placenta tissue lysates, Lane 5: human A549 whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFATC4 antigen affinity purified polyclonal antibody (Catalog # A02599-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFATC4 at approximately 95120KD. The expected band size for NFATC4 is at 95KD.
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