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NFIA/NFIB/NFIC/NFIX Antibody

PACO04425
Assay Genie
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNFIA
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Overview

  • Supplier
    Assay Genie
  • Product Name
    NFIA/NFIB/NFIC/NFIX Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHC, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4774
  • Target name
    NFIA
  • Target description
    nuclear factor I A
  • Target synonyms
    BRMUTD, C1DELp32p31, CTF, DEL1P32P31, NF-I/A, NF1-A, NFI-A, NFI-L, nuclear factor 1 A-type, CCAAT-box-binding transcription factor, TGGCA-binding protein
  • Host
    Rabbit
  • Protein IDO00712
  • Protein Name
    Nuclear factor 1 B-type
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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MSDS

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Figure 1. Western blot analysis of NFIA using anti-NFIA antibody (A03531). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse L929 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIA antigen affinity purified polyclonal antibody (Catalog # A03531) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFIA at approximately 70 kDa. The expected band size for NFIA is at 56 kDa.
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Immunohistochemical staining of human cerebral cortex, gastrointestinal, lymphoid tissues and testis using Anti-NFIA antibody HPA006111 (A) shows similar protein distribution across tissues to independent antibody HPA008884 (B).
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NFIA antibody detects NFIA protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFIA protein stained by NFIA antibody (GTX114580) diluted at 1:1000. Blue: Hoechst 33342 staining.
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