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NFKB1 Polyclonal Antibody

CAC14122
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNFKB1
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Overview

  • Supplier
    Biomatik
  • Product Name
    NFKB1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4790
  • Target name
    NFKB1
  • Target description
    nuclear factor kappa B subunit 1
  • Target synonyms
    CVID12; DNA-binding factor KBF1; EBP-1; KBF1; NFkappaB; NF-kappaB; NF-kappa-B1; NF-kappabeta; NF-kB; NF-kB1; NFKB-p105; NFKB-p50; nuclear factor kappa-B DNA binding subunit; nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19838
  • Protein Name
    Nuclear factor NF-kappa-B p105 subunit
  • Scientific Description
    The NFKB1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF, ChIP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody (PB9087). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NF-kB p65/RELA antigen affinity purified polyclonal antibody (Catalog # PB9087) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NF-kB p65/RELA at approximately 65 kDa. The expected band size for NF-kB p65/RELA is at 60 kDa.
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