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NFKBIZ / IKBZ Antibody

LS-C832630
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNFKBIZ
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NFKBIZ / IKBZ Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:10000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.44 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID64332
  • Target name
    NFKBIZ
  • Target description
    NFKB inhibitor zeta
  • Target synonyms
    I-kappa-B-zeta, IKBZ, INAP, IkappaB-zeta, MAIL, ikB-zeta, ikappaBzeta, NF-kappa-B inhibitor zeta, IL-1 inducible nuclear ankyrin-repeat protein, molecule possessing ankyrin repeats induced by lipopolysaccharide, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFKBIZ using anti-NFKBIZ antibody (A05312-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFKBIZ antigen affinity purified polyclonal antibody (Catalog # A05312-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFKBIZ at approximately 85 kDa. The expected band size for NFKBIZ is at 68 kDa.
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