Immunofluorescence staining of PC-3 cells with CSB-PA850809LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of PC-3 cells with CSB-PA850809LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
NGEF Antibody
CSB-PA850809LA01HU
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetNGEF
Overview
- SupplierCusabio
- Product NameNGEF Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID25791
- Target nameNGEF
- Target descriptionneuronal guanine nucleotide exchange factor
- Target synonymsARHGEF27; EPHEXIN; ephexin1; ephexin-1; eph-interacting exchange protein
- HostRabbit
- IsotypeIgG
- Protein IDQ8N5V2
- Protein NameEphexin-1
- Scientific DescriptionActs as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203