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NIP7 antibody detects NIP7 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE NIP7 antibody (GTX120926) dilution: 1:1000
NIP7 antibody detects NIP7 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE NIP7 antibody (GTX120926) dilution: 1:1000
NIP7 antibody detects NIP7 protein by Western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE NIP7 antibody (GTX120926) dilution: 1:1000

NIP7 antibody

GTX120926
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNIP7
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Overview

  • Supplier
    GeneTex
  • Product Name
    NIP7 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51388
  • Target name
    NIP7
  • Target description
    nucleolar pre-rRNA processing protein NIP7
  • Target synonyms
    CGI-37, HSPC031, KD93, 60S ribosome subunit biogenesis protein NIP7 homolog, NIP7, nucleolar pre-rRNA processing protein, nuclear import 7 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y221
  • Protein Name
    60S ribosome subunit biogenesis protein NIP7 homolog
  • Scientific Description
    Required for proper 27S pre-rRNA processing and 60S ribosome subunit assembly.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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Figure 1. Western blot analysis of NIP7 using anti-NIP7 antibody (A08120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NIP7 antigen affinity purified polyclonal antibody (Catalog # A08120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NIP7 at approximately 20 kDa. The expected band size for NIP7 is at 20 kDa.
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