NK Cell Subculture Medium

Cosmo Bio USA

NK Cell Subculture Medium

16

Research Use Only

Composition: NK Cell Initial Flask NK Cell Initial Medium NK Cell Initial Cocktail NK Cell Subculture Medium [Other supplies required] Ficoll-Paque (GE Healthcare, Sweden) Sterile PBS FBS or autologous plasma (It is desirable to be heat-inactivated at 56°C for 30 minutes.) Sterile conical centrifuge tubes Natural killer (NK) cells can be expanded from human peripheral blood mononuclear cells (PBMCs) without using feeder cells. NK cells can be expanded several hundred to several thousand-fold by 2 - 3 weeks of culture. One kit issufficient toexpand NK cells from 20 - 50 ml of whole blood.

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