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Western Blot Positive WB detected in: Rat heart tissue All lanes: NKD1 antibody at 1.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: Rat heart tissue All lanes: NKD1 antibody at 1.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western Blot Positive WB detected in: Rat heart tissue All lanes: NKD1 antibody at 1.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa

NKD1 Antibody

CSB-PA853369LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetNKD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NKD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID85407
  • Target name
    NKD1
  • Target description
    NKD inhibitor of WNT signaling pathway 1
  • Target synonyms
    Dvl-binding protein; naked cuticle homolog 1; naked cuticle-1; Naked1; naked-1; NKD1, WNT signaling pathway inhibitor; protein naked cuticle homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969G9
  • Protein Name
    Protein naked cuticle homolog 1
  • Scientific Description
    Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NKD1 using anti-NKD1 antibody (A06747-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NKD1 antigen affinity purified polyclonal antibody (Catalog # A06747-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NKD1 at approximately 52 kDa. The expected band size for NKD1 is at 52 kDa.
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