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WB analysis of COS cell lysates using GTX87095 NLE1 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of COS cell lysates using GTX87095 NLE1 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of COS cell lysates using GTX87095 NLE1 antibody. The lane on the right is blocked with the synthesized peptide.

NLE1 antibody

GTX87095
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetNLE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    NLE1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54475
  • Target name
    NLE1
  • Target description
    notchless homolog 1
  • Target synonyms
    NLE; Notchless gene homolog; notchless protein homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NVX2
  • Protein Name
    Notchless protein homolog 1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NLE1 using anti-NLE1 antibody (A09529-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat ovary tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLE1 antigen affinity purified polyclonal antibody (A09529-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLE1 at approximately 45 kDa. The expected band size for NLE1 is at 53 kDa.
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