NM LYSE: Flow Cytometry Lysing Solution (CE/IVD)
GAS-003-1-CE/IVD
Product group Technique Specific Products
Overview
- SupplierNordic-MUbio
- Product NameNM LYSE: Flow Cytometry Lysing Solution (CE/IVD)
- Delivery Days Customer7
- Application Supplier NoteNo-Wash Staining and Lysing Procedure - For each sample add 50 microl of EDTA anti-coagulated blood to a 3-5 ml tube - Add 20 microl of the appropriate Nordic-MUbio monoclonal antibody conjugate - Incubate the tube for 15 minutes at 4°C or at room temperature in the dark - Add 100 microl NM-LYSE to each tube and incubate for 10 minutes at room temperature - Add 1 ml of destilled water and vortex, incubate for 5-10 minutes at room temperature - Analyze immediately or store samples at 2-8° C in the dark and analyze within 24 hours Wash Staining and Lysing Procedure - For each sample add 50 microl of EDTA anti-coagulated blood to a 3-5 ml tube - Add 20 microl of the appropriate Nordic-MUbio monoclonal antibody conjugate - Incubate the tube for 15 minutes at 4°C or at room temperature in the dark - Add 100 microl NM-LYSE to each tube and incubate for 10 minutes at room temperature - Add 3-4 ml of destilled water and vortex, incubate for 5-10 minutes at room temperature - Centrifuge tube for 5 minutes at 300 g - Aspirate supernatant and resuspend pellet in 0.3 ml of sheath fluid - Analyze immediately or store samples at 2-8° C in the dark and analyze within 24 hours
- ApplicationsFlow Cytometry
- Category SupplierBuffers and reagents
- CertificationResearch Use Only
- Storage InstructionRT
- UNSPSC41116148
References
- 1. Bossuyt, X., Marti, G. E. & Fleisher, T. A. (1997) Cytometry 30, 124-33. 2. Fritsch, G., Printz, D., Stimpfl, M., Dworzak, M. N., Witt, V., Potschger, U. & Buchinger, P. (1997) Transfusion 37, 775-84. 3. Kormoczi, G. F., Wolfel, U. M., Rosenkranz, A. R., Horl, W. H., Oberbauer, R. & Zlabinger, G. J. (2001) J Immunol 167, 451-60. 4. Menendez, P., Redondo, O., Rodriguez, A., Lopez-Berges, M. C., Ercilla, G., Lopez, A., Duran, A., Almeida, J., Perez-Simon, J. A., San Miguel, J. F., Gratama, J. W. & Orfao, A. (1998) Cytometry 34, 264-71.