NM LYSE: Flow Cytometry Lysing Solution

Nordic-MUbio

NM LYSE: Flow Cytometry Lysing Solution

7

No-Wash Staining and Lysing Procedure - For each sample add 50 microl of EDTA anti-coagulated blood to a 3-5 ml tube - Add 20 microl of the appropriate Nordic-MUbio monoclonal antibody conjugate - Incubate the tube for 15 minutes at 4°C or at room temperature in the dark - Add 100 microl NM-LYSE to each tube and incubate for 10 minutes at room temperature - Add 1 ml of destilled water and vortex, incubate for 5-10 minutes at room temperature - Analyze immediately or store samples at 2-8° C in the dark and analyze within 24 hours Wash Staining and Lysing Procedure - For each sample add 50 microl of EDTA anti-coagulated blood to a 3-5 ml tube - Add 20 microl of the appropriate Nordic-MUbio monoclonal antibody conjugate - Incubate the tube for 15 minutes at 4°C or at room temperature in the dark - Add 100 microl NM-LYSE to each tube and incubate for 10 minutes at room temperature - Add 3-4 ml of destilled water and vortex, incubate for 5-10 minutes at room temperature - Centrifuge tube for 5 minutes at 300 g - Aspirate supernatant and resuspend pellet in 0.3 ml of sheath fluid - Analyze immediately or store samples at 2-8° C in the dark and analyze within 24 hours

Flow Cytometry

Buffers and reagents

Research Use Only

RT

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