
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NMDAR2A antibody (GTX103558) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NMDAR2A antibody
GTX103558
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRIN2A
Overview
- SupplierGeneTex
- Product NameNMDAR2A antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2903
- Target nameGRIN2A
- Target descriptionglutamate ionotropic receptor NMDA type subunit 2A
- Target synonymsEPND, FESD, GluN2A, LKS, NMDAR2A, NR2A, glutamate receptor ionotropic, NMDA 2A, N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor channel, subunit epsilon-1, N-methyl-D-aspartate receptor subunit 2A, glutamate ionotropic receptor NMDA 2A, glutamate receptor, ionotropic, N-methyl D-aspartate 2A
- HostRabbit
- IsotypeIgG
- Protein IDQ12879
- Protein NameGlutamate receptor ionotropic, NMDA 2A
- Scientific DescriptionN-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203

![NMDAR2A antibody detects NMDAR2A protein at cell membrane by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR2A stained by NMDAR2A antibody (GTX103558) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). NMDAR2A antibody detects NMDAR2A protein at cell membrane by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR2A stained by NMDAR2A antibody (GTX103558) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX103558/GTX103558_43915_20221209_ICC_IF_R_22122018_297.webp)

![NMDAR2A antibody detects NMDAR2A protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR2A protein stained by NMDAR2A antibody (GTX103558) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). NMDAR2A antibody detects NMDAR2A protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMDAR2A protein stained by NMDAR2A antibody (GTX103558) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX103558/GTX103558_40394_20170719_IFA_R_w_23060119_258.webp)

![NMDAR2A antibody [C1C2], Internal detects GRIN2A protein at cytosol and nucleus on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. NMDAR2A antibody [C1C2], Internal (GTX113717) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX113717/GTX113717_40163_IHC_w_23060501_819.webp)



