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A431 cell lysates probed with NMI (8C9) Polyclonal Antibody, unconjugated (bsm-51329M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
A431 cell lysates probed with NMI (8C9) Polyclonal Antibody, unconjugated (bsm-51329M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
A431 cell lysates probed with NMI (8C9) Polyclonal Antibody, unconjugated (bsm-51329M) at 1:2000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

NMI (8C9) Monoclonal Antibody

BSM-51329M
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetNMI
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    NMI (8C9) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8C9
  • Concentration
    0.5 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID9111
  • Target name
    NMI
  • Target description
    N-myc and STAT interactor
  • Target synonyms
    N-myc-interactor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13287
  • Protein Name
    N-myc-interactor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NMI using anti-NMI antibody (PB9340). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMI antigen affinity purified polyclonal antibody (Catalog # PB9340) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMI at approximately 35KD. The expected band size for NMI is at 35KD.
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