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Western Blot analysis of HepG2 cells using Neuromedin-U Polyclonal Antibody
Western Blot analysis of HepG2 cells using Neuromedin-U Polyclonal Antibody
Western Blot analysis of HepG2 cells using Neuromedin-U Polyclonal Antibody

NMU Antibody

CSB-PA003417
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNMU
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Overview

  • Supplier
    Cusabio
  • Product Name
    NMU Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10874
  • Target name
    NMU
  • Target description
    neuromedin U
  • Target synonyms
    neuromedin U precursor-related peptide/neuromedin U preproprotein; neuromedin-U; prepro-NMU
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48645
  • Protein Name
    Neuromedin-U
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of cells in the peripheral ganglion.
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Figure 1. Western blot analysis of NMU using anti-NMU antibody (A01919-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMU antigen affinity purified polyclonal antibody (Catalog # A01919-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMU at approximately 26KD. The expected band size for NMU is at 20KD.
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Anti-NMU Antibody Picoband(r)A01919-1-CARRIER-FREE
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