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WB analysis of various sample lysates using GTX32754 NNMT antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32754 NNMT antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32754 NNMT antibody. Dilution : 1:1000 Loading : 25microg per lane

NNMT antibody

GTX32754
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNNMT
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Overview

  • Supplier
    GeneTex
  • Product Name
    NNMT antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4837
  • Target name
    NNMT
  • Target description
    nicotinamide N-methyltransferase
  • Target synonyms
    nicotinamide N-methyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40261
  • Protein Name
    Nicotinamide N-methyltransferase
  • Scientific Description
    N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: NNMT antibody at 2.47microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 30 kDa Observed band size: 30 kDa
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Figure 1. Western blot analysis of NNMT using anti-NNMT antibody (A02676-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NNMT antigen affinity purified polyclonal antibody (Catalog # A02676-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NNMT at approximately 28 kDa. The expected band size for NNMT is at 30 kDa.
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