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ProductsBack/NNP-1 (A-6): m-IgG Fc BP-HRP Bundle

NNP-1 (A-6): m-IgG Fc BP-HRP Bundle

SC-527838
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetRRP1
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    NNP-1 (A-6): m-IgG Fc BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8568
  • Target name
    RRP1
  • Target description
    ribosomal RNA processing 1
  • Target synonyms
    D21S2056E, NNP-1, NOP52, RRP1A, ribosomal RNA processing protein 1 homolog A, Nnp1 homolog, nucleolar protein, RRP1-like protein, novel nuclear protein 1, nucleolar protein Nop52, protein NNP-1, ribosomal RNA processing 1 homolog
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRP1 using anti-RRP1 antibody (A06230-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP1 antigen affinity purified polyclonal antibody (Catalog # A06230-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP1 at approximately 53 kDa. The expected band size for RRP1 is at 53 kDa.
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