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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA015917LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA015917LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA015917LA01HU at dilution of 1:100

NOG Antibody

CSB-PA015917LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNOG
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Overview

  • Supplier
    Cusabio
  • Product Name
    NOG Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9241
  • Target name
    NOG
  • Target description
    noggin
  • Target synonyms
    noggin; SYM1; symphalangism 1 (proximal); SYNS1; SYNS1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13253
  • Protein Name
    Noggin
  • Scientific Description
    Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. Essential for cartilage morphogenesis and joint formation. Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (PubMed:21976273, PubMed:26643732).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Noggin using anti-Noggin antibody (A04448-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A375 whole cell lysates, Lane 5: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Noggin antigen affinity purified polyclonal antibody (Catalog # A04448-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Noggin at approximately 26KD. The expected band size for Noggin is at 26KD.
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